gal4 ad Search Results


gal4 ad monoclonal antibody  (TaKaRa)
93
TaKaRa gal4 ad monoclonal antibody
Gal4 Ad Monoclonal Antibody, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gal4 ad monoclonal antibody/product/TaKaRa
Average 93 stars, based on 1 article reviews
gal4 ad monoclonal antibody - by Bioz Stars, 2026-03
93/100 stars
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gal4 activation domain  (Dualsystems Biotech)
90
Dualsystems Biotech gal4 activation domain
(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the <t>Gal4</t> activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.
Gal4 Activation Domain, supplied by Dualsystems Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gal4 activation domain/product/Dualsystems Biotech
Average 90 stars, based on 1 article reviews
gal4 activation domain - by Bioz Stars, 2026-03
90/100 stars
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lexa or gal4 fusions to lst8  (Hybrigenics sa)
90
Hybrigenics sa lexa or gal4 fusions to lst8
(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the <t>Gal4</t> activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.
Lexa Or Gal4 Fusions To Lst8, supplied by Hybrigenics sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lexa or gal4 fusions to lst8/product/Hybrigenics sa
Average 90 stars, based on 1 article reviews
lexa or gal4 fusions to lst8 - by Bioz Stars, 2026-03
90/100 stars
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gal4-ad vectors  (SLIT2 LTD)
90
SLIT2 LTD gal4-ad vectors
(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the <t>Gal4</t> activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.
Gal4 Ad Vectors, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gal4-ad vectors/product/SLIT2 LTD
Average 90 stars, based on 1 article reviews
gal4-ad vectors - by Bioz Stars, 2026-03
90/100 stars
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anti-gal4 ad antibody ym3410  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company anti-gal4 ad antibody ym3410
(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the <t>Gal4</t> activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.
Anti Gal4 Ad Antibody Ym3410, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-gal4 ad antibody ym3410/product/ImmunoWay Biotechnology Company
Average 90 stars, based on 1 article reviews
anti-gal4 ad antibody ym3410 - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


(A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the Gal4 activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.

Journal: PLoS ONE

Article Title: Histidine Domain-Protein Tyrosine Phosphatase Interacts with Grb2 and GrpL

doi: 10.1371/journal.pone.0014339

Figure Lengend Snippet: (A) A scheme of the yeast two-hybrid screen. The binding of the “prey” to the HD-PTP “bait” yields a functional transcription factor that promotes the transcription of the reporter genes. In the absence of an interaction between the “bait” and the “prey” the reporters (e.g., HIS3 and LacZ ) are not induced. (B) Yeast L40 cells were co-transformed with the expression plasmid encoding the full-length HD-PTP fused with LexA DNA-binding domain (DBD, bait) and each expression plasmid encoding either the full length GrpL or the C-terminal half of Grb2 fused with the Gal4 activation domain (AD, prey). Transformants were selected on double dropout (SD-Trp, -Leu) medium plate. Each positive transformant was streaked on double dropout selection medium plate (SD-Trp-Leu) and quadruple dropout (SD-Trp,-Leu,-His,-Met). A filter replica of the SD-Trp,-Leu,-His,-Met plate was used to assay for β-galactosidase activity. Ctrl, empty vectors. (C) Schematic representation of the domain structure of Grb2, GrpL and the two interacting fragments found by the yeast two-hybrid screening.

Article Snippet: Yeast two hybrid screening was performed using a human adult colon cDNA library in pGAD-DS, with selectable marker Leu and a GAL4 activation domain (Dualsystems Biotech).

Techniques: Two Hybrid Screening, Binding Assay, Functional Assay, Transformation Assay, Expressing, Plasmid Preparation, Activation Assay, Selection, Activity Assay